Twinkle Snow is the fifth zone in Sonic Advance 3, and is the customary ice level, though it possesses surprisingly few "snow" gimmicks apart from thick snow that slows characters down. The northern lights can be seen in the skies at the top of the zone.
Mine carts offer a quick ride through portions of this zone, along with ice machines that will suck up characters, then launch them in a frozen state (though freezing in this game is harmless). There are also platforms that the player can interact with by holding to make it sink slowly (and will sink faster if both characters are crouching on it), then release to be catapulted into the air. Water is present within the zone, though it is mostly avoidable by taking a high route (this also helps avoid the bottomless pits, something many levels in Sonic Advance 3 have); however, Act 2 has a mandatory water section near the end.
- Ginpe - A penguin robot that waddles back and forth and shoots projectiles from its top hat.
- Hariisen - A pufferfish badnik found in underwater areas. Puffs up and fires spikes in six directions when approached.
- Yadokka - A hermit crab robot found in underwater areas. Fires its shell like a rocket similar to the Turbo Spiker.
- Yukigasen - A snowman robot that remains stationary and lobs snowballs like a pitching machine.