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General Comparisons
The player does not run in at the beginning.
The overscan border color for Act 1 is red, instead of black like the other zones.
The light beam transportation systems have a slightly different countdown tick. It's four low tones, instead of three low tones and one high tone.
The final boss music still doesn't play. DEZ Act 2's theme just continues to play.
If Death Egg's final boss is defeated while it's firing its laser, the laser stays on screen while it explodes.
After the final boss machine collapses completely, everything stops rumbling.
The final chase is slower, and Eggman comes back on screen more slowly.
After defeating Eggman during the final chase, the HUD turns green. This is because the game has loaded Knuckles' 1P palette into the Knuckles CP palette slot.
After clearing the final Death Egg Zone boss, the game attempts to enter Sonic 3's credits sequence, as was the case with the 0517 prototype of Sonic 3C. However, the screen mode for this sequence no longer exists, so the game softlocks.
Act 1
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0525 prototype
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Final game
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Act 2
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0525 prototype
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Final game
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References